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LTBP1 Polyclonal antibody

LTBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22065-1-AP

Synonyms

LTBP 1, LTBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using LTBP1 Polyclonal antibody (22065-1-AP)

    WB analysis of mouse liver using 22065-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22065-1-AP (LTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LTBP1 Polyclonal antibody (22065-1-AP)

    IHC staining of human breast cancer using 22065-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22065-1-AP (LTBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LTBP1 Polyclonal antibody (22065-1-AP)

    IHC staining of human breast cancer using 22065-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22065-1-AP (LTBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22065-1-AP targets LTBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LTBP1 fusion protein Ag17141
Full Name latent transforming growth factor beta binding protein 1
Calculated Molecular Weight 1721 aa, 187 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC130289
Gene Symbol LTBP1
Gene ID (NCBI) 4052
RRIDAB_11232219
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LTBP1 antibody 22065-1-APDownload protocol
IHC protocol for LTBP1 antibody 22065-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols