LTA4H Polyclonal antibody

LTA4H Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13662-1-AP

Synonyms

Leukotriene A 4 hydrolase, Leukotriene A(4) hydrolase, leukotriene A4 hydrolase, LTA 4 hydrolase, LTA4, LTA4H

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Validation Data Gallery

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  WB analysis of HEK-293 using 13662-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13662-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13662-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13662-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13662-1-AP

  IP result of anti-LTA4H (IP:13662-1-AP, 4ug; Detection:13662-1-AP 1:500) with HEK-293 cells lysate 1120ug.

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  IHC staining of human skin using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human skin tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

13662-1-AP targets LTA4H in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LTA4H fusion protein Ag4568
• Full Name: leukotriene A4 hydrolase
• Calculated Molecular Weight: 611 aa, 69 kDa
• Observed Molecular Weight: 57-70 kDa
• GenBank Accession Number: BC032528
• Gene Symbol: LTA4H
• Gene ID (NCBI): 4048
• RRID: AB_2138747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LTA4H(Leukotriene A-4 hydrolase), also named as LTA4, is essential for the conversion of LTA4 to LTB4, an inflammatory lipid mediator(PMID:10601658). LTA4H also possesses aminopeptidase activity that it can degrade PGP, which is a biomarker for chronic obstructive pulmonary disease and is implicated in neutrophil persistence in the lung(PMID:20813919). This protein has 4 isoforms produced by alternative splicing with the MW of 69, 59, 57, 67 kDa.

Publications

Species	Application	Title
human	WB	Sci Signal; ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation in rheumatoid arthritis; Authors - Hao Cai
human	WB	Proteomics; Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma.; Authors - Wei Gao
mouse	IF	JHEP Rep; Time-resolved single-cell transcriptomics reveals the landscape and dynamics of hepatic cells in sepsis-induced acute liver dysfunction; Authors - Gan Chen