LSS Polyclonal antibody

LSS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 13715-1-AP

Synonyms

Oxidosqualene--lanosterol cyclase, OSC, Lanosterol synthase, hOSC, EC:5.4.99.7

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Validation Data Gallery

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  WB analysis using 13715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13715-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13715-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13715-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 13715-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13715-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13715-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13715-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13715-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13715-1-AP

  IP result of anti-LSS (IP:13715-1-AP, 3ug; Detection:13715-1-AP 1:500) with HepG2 cells lysate 2900ug.

• 

  IHC staining of human liver using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse testis using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13715-1-AP

  Immunofluorescent analysis of HepG2 cells using 13715-1-AP (LSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HT-1376 cells, PC-3 cells, HeLa cells, HepG2 cells, mouse testis tissue, human brain tissue, mouse pancreas tissue, mouse liver tissue, SH-SY5Y cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13715-1-AP targets LSS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LSS fusion protein Ag4655
• Full Name: lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
• Calculated Molecular Weight: 732 aa, 83 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC035638
• Gene Symbol: LSS
• Gene ID (NCBI): 4047
• RRID: AB_10597096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LSS, also named as OSC, is lanosterol synthase which catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. LSS gene has three isoforms with MW 74 kDa and 82-85 kDa. This antibody can recognize all these three isoforms.

Publications

Species	Application	Title
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	WB	J Invest Dermatol; Bi-allelic Variants in LSS Cause Palmoplantar Keratoderma-Congenital Alopecia Syndrome Type 2.; Authors - Fang Yang
human	WB,IHC	PLoS Genet; Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice.; Authors - Yoichi Wada
rat	WB	J Biol Chem; The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.; Authors - Lee G D Fryer
human	WB, IHC	Phytomedicine; Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.; Authors - Xing Yue Xu
human	WB	J Biol Chem; The antiviral enzyme viperin inhibits cholesterol biosynthesis.; Authors - Timothy J Grunkemeyer