LRWD1 Polyclonal antibody

LRWD1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19546-1-AP

Synonyms

LRWD1

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Validation Data Gallery

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  WB analysis using 19546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19546-1-AP (LRWD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19546-1-AP (LRWD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 19546-1-AP

  IP result of anti-LRWD1 (IP:19546-1-AP, 4ug; Detection:19546-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

19546-1-AP targets LRWD1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: leucine-rich repeats and WD repeat domain containing 1
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: NM_152892
• Gene Symbol: LRWD1
• Gene ID (NCBI): 222229
• RRID: AB_2878588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRWD1 contains 3 LRR (leucine-rich) repeats and 5 WD repeats. LRWD1 is phosphorylated upon DNA damage by ATM or ATR. The antibody recognize the N-term of LRWD1.

Publications

Species	Application	Title
human	WB	Development; PRSS50 is a testis protease responsible for proper sperm tail formation and function.; Authors - Jason M Scovell