LRRC8A/SWELL1 Polyclonal antibody

LRRC8A/SWELL1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17155-1-AP

Synonyms

LRRC8A, SWELL1, LRRC8, Leucine-rich repeat-containing protein 8A, KIAA1437

---

Validation Data Gallery

• 

  WB analysis using 17155-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 17155-1-AP (LRRC8A/SWELL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17155-1-AP

  IP result of anti-LRRC8A/SWELL1 (IP:17155-1-AP, 4ug; Detection:17155-1-AP 1:800) with mouse brain tissue lysate 2100 ug.

• 

  IHC staining of human colon cancer using 17155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17155-1-AP (LRRC8A/SWELL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17155-1-AP (LRRC8A/SWELL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: rat lung tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

17155-1-AP targets LRRC8A/SWELL1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRRC8A/SWELL1 fusion protein Ag10062
• Full Name: leucine rich repeat containing 8 family, member A
• Calculated Molecular Weight: 810 aa, 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC051322
• Gene Symbol: LRRC8A
• Gene ID (NCBI): 56262
• RRID: AB_2918050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRC8A, also named as SWELL1, KIAA1437 and LRRC8, belongs to the LRRC8 family. LRRC8A is a ubiquitous and integral component of the VRAC and has been reported to be required for regulation of cell volume. It indicates that LRRC8A plays a role in regulating the flow of ions, especially anions, into and out of cells (PMID: 32079488). Additionally, there have been several reports that LRRC8A is critical for differentiation of adipocytes and lymphocytes (PMID: 31323188). The molecular weight of LRRC8A is 94 kDa.

Publications

Species	Application	Title
human	IHC	Int J Biol Sci; Upregulation of LRRC8A by m5C modification-mediated mRNA stability suppresses apoptosis and facilitates tumorigenesis in cervical cancer; Authors - Yanjie Chen