LRRC4C Polyclonal antibody

LRRC4C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13845-1-AP

Synonyms

KIAA1580, LRRC4C, Netrin G1 ligand, NGL 1, NGL1

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Validation Data Gallery

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  WB analysis of Y79 using 13845-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13845-1-AP (LRRC4C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 13845-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13845-1-AP (LRRC4C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 13845-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13845-1-AP (LRRC4C antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Y79 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

13845-1-AP targets LRRC4C in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRRC4C fusion protein Ag4811
• Full Name: leucine rich repeat containing 4C
• Calculated Molecular Weight: 640 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC041374
• Gene Symbol: LRRC4C
• Gene ID (NCBI): 57689
• RRID: AB_2137623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Cell Rep Med; Single-cell sequencing identifies differentiation-related markers for molecular classification and recurrence prediction of PitNET; Authors - Qilin Zhang
human	IF	Mol Med; LRRC4 functions as a neuron-protective role in experimental autoimmune encephalomyelitis.; Authors - Yan Zhang