LRRC47 Recombinant antibody

LRRC47 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240741A6

Cat no : 83693-3-RR

Synonyms

Leucine-rich repeat-containing protein 47, KIAA1185, 240741A6

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Validation Data Gallery

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  WB analysis using 83693-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83693-3-RR (LRRC47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 83693-3-RR

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using LRRC47 antibody (83693-3-RR, Clone: 240741A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U2OS using 83693-3-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug LRRC47 Recombinant antibody (83693-3-RR, Clone:240741A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83693-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83693-3-RR against Human LRRC47 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: Daudi cells, U-87 MG cells, L02 cells
• Positive IF/ICC detected in: L02 cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83693-3-RR is LRRC47 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LRRC47 fusion protein Ag19736
• Full Name: leucine rich repeat containing 47
• Calculated Molecular Weight: 583 aa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC031301
• Gene Symbol: LRRC47
• Gene ID (NCBI): 57470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.