LRRC47 Polyclonal antibody

• Featured Product
• KD/KO Validated

LRRC47 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 23217-1-AP

Synonyms

Leucine-rich repeat-containing protein 47, KIAA1185

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Validation Data Gallery

• 

  WB analysis of mouse liver using 23217-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 23217-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 23217-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23217-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 23217-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 23217-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 23217-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23217-1-AP (LRRC47 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 23217-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23217-1-AP (LRRC47 Antibody) at dilution of 1:50.

• 

  IF Staining of L02 using 23217-1-AP

  Immunofluorescent analysis of L02 cells using 23217-1-AP (LRRC47 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, L02 cells, Neuro-2a cells, NIH/3T3 cells, rat liver tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

23217-1-AP targets LRRC47 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRRC47 fusion protein Ag19736
• Full Name: leucine rich repeat containing 47
• Calculated Molecular Weight: 583 aa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC031301
• Gene Symbol: LRRC47
• Gene ID (NCBI): 57470
• RRID: AB_2879234
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.

Publications

Species	Application	Title
human	WB	Nature; Structural basis for the final steps of human 40S ribosome maturation.; Authors - Michael Ameismeier ; KD Validated
human	WB	bioRxiv; LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins; Authors - James A Saba ; KD Validated
human	WB,CoIP,IHC	Cell Rep; The C9ORF72 repeat expansion alters neurodevelopment; Authors - Eric Hendricks