LRRC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LRRC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10128-2-AP

Synonyms

Leucine-rich repeat-containing protein 1, LAP and no PDZ protein, LANO adapter protein, LANO

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Validation Data Gallery

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  WB analysis using 10128-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10128-2-AP (LRRC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 10128-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10128-2-AP (LRRC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10128-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRRC1 antibody (10128-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

10128-2-AP targets LRRC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRRC1 fusion protein Ag0175
• Full Name: leucine rich repeat containing 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC003193
• Gene Symbol: LRRC1
• Gene ID (NCBI): 55227
• RRID: AB_2137458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRC1 consists of 524 amino acids including 16 leucine-rich repeats and a LAP-specific domain. It has been shown to be highly expressed in several human cancers and can participate in the malignant process of cancer cells, such as hepatocellular carcinoma, breast cancer and non-small cell lung cancer. (PMID: 36370237, PMID: 37505155)

Publications

Species	Application	Title
human	WB	J Mol Histol; LRRC1 knockdown downregulates MACF1 to inhibit the malignant progression of acute myeloid leukemia by inactivating β-catenin/c-Myc signaling; Authors - Yao Wang ; KD Validated
human,mouse,rat	WB,IHC	Int J Mol Sci; The Upregulation of Leucine-Rich Repeat Containing 1 Expression Activates Hepatic Stellate Cells and Promotes Liver Fibrosis by Stabilizing Phosphorylated Smad2/3; Authors - Yake Wang ; KD Validated