LRP4 Recombinant antibody

LRP4 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242157D11

Cat no : 84973-1-RR

Synonyms

LRP-4, LRP10, LRP 4, Low-density lipoprotein receptor-related protein 4, KIAA0816

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Validation Data Gallery

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  WB analysis using 84973-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84973-1-RR (LRP4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84973-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84973-1-RR against Human LRP4 were performed. The affinity constant is 70.0 nM.

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84973-1-RR targets LRP4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LRP4 fusion protein Ag19413
• Full Name: low density lipoprotein receptor-related protein 4
• Calculated Molecular Weight: 1905 aa, 212 kDa
• Observed Molecular Weight: 245 kDa
• GenBank Accession Number: BC136667
• Gene Symbol: LRP4
• Gene ID (NCBI): 4038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRP4 (low-density lipoprotein receptor-related protein 4), also known as multiple epidermal growth factor-like domains 7 (MEGF7), is a type I single transmembrane protein of the low-density lipoprotein receptor (LDLR) family. LRP4 is composed of a large extracellular region containing multiple LDLa repeats, EGF-like domains and β-propeller domains, a transmembrane region and a cytoplasmic region containing an NPxY motif and a PDZ-interacting motif (PMID: 26071838). LRP4 binds agrin and recruits it to the MuSK signaling complex, which is critical for neuromuscular junction formation (PMID: 17119023; 18957220). LPR4 mutation or malfunction is implicated in disorders including congenital myasthenic syndrome, myasthenia gravis, and diseases of bone or kidney (26071838).