LRMP Polyclonal antibody

LRMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 19498-1-AP

Synonyms

Protein Jaw1, Processed inositol 1,4,5-triphosphate receptor associated 2, Lymphoid-restricted membrane protein, JAW1, IRAG2

---

Validation Data Gallery

• 

  WB analysis of Raji using 19498-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19498-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 19498-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 19498-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of Jurkat using 19498-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LRMP antibody (19498-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of Jurkat using 19498-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug LRMP Polyclonal antibody (19498-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Jurkat cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19498-1-AP targets LRMP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: lymphoid-restricted membrane protein
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_006152
• Gene Symbol: LRMP
• Gene ID (NCBI): 4033
• RRID: AB_10640430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRMP, also named as JAW1, is a lymphoid restricted integral membrane protein that is localised to the cytoplasmic face of the endoplasmic reticulum.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Sara (Verified Customer) (10-06-2024)

We tried flow cytometry (FC) with both human and mouse cells and it did now work for either. However, to be fair, the data sheet does not report FC as one of the tested applications and it also says that it does not work in mouse, so it is expected. If Proteintech could develop an anti-mouse LRMP that would be amazing, we haven't found any that works.

• Applications: Flow Cytometry
• Cell Tissue Type: Human and mouse