LPXN Recombinant antibody
LPXN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240483G10
Cat no : 83657-5-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Ramos cells, Jurkat cells, MDA-MB-231 cells |
Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83657-5-RR is LPXN Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | LPXN fusion protein Ag34956 |
Full Name | leupaxin |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC019035 |
Gene Symbol | LPXN |
Gene ID (NCBI) | 9404 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).
Protocols
Product Specific Protocols | |
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WB protocol for LPXN antibody 83657-5-RR | Download protocol |
IF protocol for LPXN antibody 83657-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |