LPXN Polyclonal antibody
LPXN Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 31231-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MDA-MB-231 cells, PC-3 cells, RAW 264.7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
31231-1-AP targets LPXN in WB, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LPXN fusion protein Ag34956 |
Full Name | leupaxin |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 40-50 kDa |
GenBank Accession Number | BC019035 |
Gene Symbol | LPXN |
Gene ID (NCBI) | 9404 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).
Protocols
Product Specific Protocols | |
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WB protocol for LPXN antibody 31231-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |