LPCAT2 Monoclonal antibody
LPCAT2 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1E6G1
Cat no : 68476-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | BxPC-3 cells, A375 cells, A549 cells, HEK-293 cells, HT-29 cells, Jurkat cells, HL-60 cells, HSC-T6 cells, NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68476-1-Ig targets LPCAT2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | LPCAT2 fusion protein Ag26420 |
Full Name | lysophosphatidylcholine acyltransferase 2 |
Calculated Molecular Weight | 60 kDa |
Observed Molecular Weight | 68 kDa |
GenBank Accession Number | BC002472 |
Gene Symbol | LPCAT2 |
Gene ID (NCBI) | 54947 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LPCAT2 (Lysophosphatidylcholine acyltransferase 2) is also named as AGPAT11 and AYTL1. Lipid droplet (LD) content of CRC cells positively correlates with the expression of lysophosphatidylcholine acyltransferase 2 (LPCAT2), an LD-localised enzyme supporting phosphatidylcholine synthesis (PMID: 29358673). Mitochondria play a significant role in ACSL4/LPCAT2-driven ferroptosis (PMID: 3762584). In response to LPS, LPCAT2, but not LPCAT1, rapidly associates with TLR4 and translocates to membrane lipid raft domains to regulate macrophage inflammatory gene expression (PMID: 32587324).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for LPCAT2 antibody 68476-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |