LOXL2 Monoclonal antibody

LOXL2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

1H7F1

Reviews (1)

Cat no : 67139-1-Ig

Synonyms

LOR2, LOXL2, Lysyl oxidase homolog 2, lysyl oxidase like 2, Lysyl oxidase like protein 2, WS9 14

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Validation Data Gallery

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  WB analysis of A549 using 67139-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 67139-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67139-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 67139-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat colon using 67139-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67139-1-Ig (LOXL2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 67139-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67139-1-Ig (LOXL2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67139-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67139-1-Ig (LOXL2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, human placenta tissue, A375 cells, human heart tissue
• Positive IHC detected in: rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

67139-1-Ig targets LOXL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LOXL2 fusion protein Ag28566
• Full Name: lysyl oxidase-like 2
• Calculated Molecular Weight: 774 aa, 87 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC000594
• Gene Symbol: LOXL2
• Gene ID (NCBI): 4017
• RRID: AB_2882438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. We detected a band about 100 kd which is a secretory mature LOXL2 protein modified by glycosylation (PMID: 24014025).

Publications

Species	Application	Title
human		J Clin Invest; LOXL2-induced PEAR1 Ser891 phosphorylation suppresses CD44 degradation and promotes triple-negative breast cancer metastasis; Authors - Yingzhi Shen
mouse	WB	Aging Cell; Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.; Authors - Yongxin Wu
mouse	WB,IF	Int J Biol Macromol; Depletion of LOXL2 improves respiratory capacity: From air-breathing fish to mammal under hypoxia.; Authors - Bing Sun
human	WB	Oncogene; METTL1/WDR4-mediated tRNA m7G modification and mRNA translation control promote oncogenesis and doxorubicin resistance; Authors - Zhaoyu Wang
human	IF	Neuron; Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier; Authors - Yuan Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (10-04-2021)

The calculated MW is 87 kd. This antibody recognized major bands at 65, and 60 kd.

• Applications: Western Blot