LONP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

LONP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18035-1-AP

Synonyms

LONPL, LONP, Lon protease-like protein 2, Lon protease homolog 2, peroxisomal, Lon protease 2

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Validation Data Gallery

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  WB analysis of Jurkat using 18035-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18035-1-AP (LONP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 18035-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18035-1-AP (LONP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 18035-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18035-1-AP (LONP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18035-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18035-1-AP (LONP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

18035-1-AP targets LONP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LONP2 fusion protein Ag12650
• Full Name: lon peptidase 2, peroxisomal
• Calculated Molecular Weight: 852 aa, 95 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC093910
• Gene Symbol: LONP2
• Gene ID (NCBI): 83752
• RRID: AB_2878485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,WB	Gene; Role of LONP2 in head and neck squamous cell carcinoma; Authors - Xiaobo Cui ; KD Validated