LMX1B Polyclonal antibody

• Featured Product
• KD/KO Validated

LMX1B Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 18278-1-AP

Synonyms

LIM/homeobox protein 1.2, LIM/homeobox protein LMX1B, LMX 1.2, LMX1.2, LMX1B

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Validation Data Gallery

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  WB analysis of HeLa using 18278-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18278-1-AP (LMX1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of embryonic kidney tissue using 18278-1-AP

  IF result of anti-LMX1B (18278-1-AP ,1:900) with embryonic kidney by Dr. Joo-Seop Park. Red: Podocytes, anti-rabbir Alexa555; Blue: Hoechst.

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF detected in: embryonic kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

18278-1-AP targets LMX1B in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LMX1B fusion protein Ag13096
• Full Name: LIM homeobox transcription factor 1, beta
• Calculated Molecular Weight: 372 aa, 42 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC112120
• Gene Symbol: LMX1B
• Gene ID (NCBI): 4010
• RRID: AB_11232226
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LMX1B, LIM homeodomain factor 1b, functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Lmx1b is a continuously expressed, terminal selector-type factor in 5-HT neurons. It is required for maintenance of central serotonergic neurons (PMID: 17151281, PMID: 14602809). It is expressed in brain, testis, skeletal muscle, and pancreatic islets (PMID: 35417751, PMID: 18512225).

Publications

Species	Application	Title
		Autophagy; A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons.; Authors - Petros Stathakos
rat	IHC	Sci Signal; Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.; Authors - Zi-Yang Zhang
mouse	WB	Neurobiol Dis; Maternal Herpesviridae infection during pregnancy alters midbrain dopaminergic signatures in adult offspring.; Authors - Amanda R Burmeister
human	WB	Biomed Pharmacother; LMX1B involved in the radioresistance, proliferation and migration of esophageal cancer cells.; Authors - Chunbao Zang ; KD Validated
		Methods Mol Biol; Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research.; Authors - Petros Stathakos
human	WB,IF,IP,ChIP	J Cell Biol; ATG8-dependent LMX1B-autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience; Authors - Natalia Jiménez-Moreno ; KD Validated