LMOD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LMOD1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 15117-1-AP

Synonyms

Thyroid-associated ophthalmopathy autoantigen, SM-Lmod, Leiomodin-1, Leiomodin1

---

Validation Data Gallery

• 

  WB analysis of HT-29 using 15117-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 15117-1-AP (LMOD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 15117-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 15117-1-AP (LMOD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 15117-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15117-1-AP (LMOD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 15117-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15117-1-AP (LMOD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 15117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using LMOD1 antibody (15117-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse bladder tissue, mouse uterus tissue, HT-29 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 9 publications below

Product Information

15117-1-AP targets LMOD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LMOD1 fusion protein Ag7176
• Full Name: leiomodin 1 (smooth muscle)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 85 kDa, 64 kDa
• GenBank Accession Number: BC001755
• Gene Symbol: LMOD1
• Gene ID (NCBI): 25802
• RRID: AB_2136682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.

Publications

Species	Application	Title
human	IF,WB	Gastroenterology; The Long Non-coding RNA Carmn is a Critical Regulator of Gastrointestinal Smooth Muscle Contractile Function and Motility; Authors - Xiangqin He
human	IF	Nat Genet; Single-nucleus chromatin accessibility profiling highlights regulatory mechanisms of coronary artery disease risk.; Authors - Adam W Turner
mouse	IF	Sci Transl Med; Nodding syndrome may be an autoimmune reaction to the parasitic worm Onchocerca volvulus.; Authors - Tory P Johnson
mouse	IF	Genome Biol; Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression.; Authors - Pan Gao
mouse	WB,IF	Proc Natl Acad Sci U S A; Loss of LMOD1 impairs smooth muscle cytocontractility and causes megacystis microcolon intestinal hypoperistalsis syndrome in humans and mice.; Authors - Danny Halim ; KO Validated
	WB,IF	PLoS Genet; Functional regulatory mechanism of smooth muscle cell-restricted LMOD1 coronary artery disease locus.; Authors - Vivek Nanda ; KD Validated