LIS1 Polyclonal antibody

LIS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20678-1-AP

Synonyms

LIS 1, LIS1, LIS2, Lissencephaly 1 protein, MDCR, MDS, PAF AH 45 kDa subunit, PAF AH alpha, PAFAH, PAFAH alpha, PAFAH1B1, PAFAHA



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20678-1-AP targets LIS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_000430
Gene Symbol LIS1
Gene ID (NCBI) 5048
RRIDAB_2878723
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet-activating factor acetylhydrolase 1B subunit alpha (LIS1), a critical mediator of neuronal migration in developing brain, is expressed throughout life. LIS1 forms a homodimer that interacts directly with the dynein complex and is essential for its enrichment at dynamic microtubule plus ends in mammalian cells. In addition to regulating the association of dynein with microtubule plus ends, LIS1 is required for efficient transport of many dynein-associated cargos and proper localization of Golgi complexes, endosomes, lysosomes, and mitochondria. The LIS1 protein has a calculated molecular mass of ~45 kDa.(PMID: 29150182, PMID: 29562183, PMID: 15800059)

Protocols

Product Specific Protocols
WB protocol for LIS1 antibody 20678-1-APDownload protocol
IHC protocol for LIS1 antibody 20678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Iran J Biotechnol

MiR-582 Down-Regulates Lissencephaly-1 (LIS1) via P-Akt and MMP-2 to Inhibit Cholangiocarcinoma Cell Proliferation and Invasion

Authors - Guochao Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Aurélie (Verified Customer) (06-13-2019)

Good antibody in WB with quite low background, 1/2000 could be enough

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse Cortex
LIS1 Antibody Western Blot, validation (1/1000 dilution) in Mouse Cortex (Cat no:20678-1-AP)