LIPI Polyclonal antibody

LIPI Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24300-1-AP

Synonyms

Cancer/testis antigen 17, CT17, Lipase member I, lipase, member I, LIPI, LPD lipase, LPDL, mPA PLA1 beta, PRED5

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Validation Data Gallery

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  WB analysis of A549 using 24300-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24300-1-AP (LIPI Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 24300-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24300-1-AP (LIPI Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24300-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24300-1-AP (LIPI antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 24300-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24300-1-AP (LIPI antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, mouse lung tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24300-1-AP is LIPI Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIPI fusion protein Ag19024
• Full Name: lipase, member I
• Calculated Molecular Weight: 460 aa, 55 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC148691
• Gene Symbol: LIPI
• Gene ID (NCBI): 149998
• RRID: AB_2879483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.