LIPH Polyclonal antibody

• Featured Product
• KD/KO Validated

LIPH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16602-1-AP

Synonyms

LPDLR, LPD lipase-related protein, lipase, member H, Lipase member H, EC:3.1.1.-

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Validation Data Gallery

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  WB analysis of A549 using 16602-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16602-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16602-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16602-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 16602-1-AP

  IP result of anti-LIPH (IP:16602-1-AP, 4ug; Detection:16602-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human pancreas using 16602-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16602-1-AP (LIPH antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 16602-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16602-1-AP (LIPH antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 16602-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 16602-1-AP (LIPH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, COLO 320 cells, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 5 publications below
• ELISA: See 1 publications below

Product Information

16602-1-AP targets LIPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIPH fusion protein Ag9775
• Full Name: lipase, member H
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC064941
• Gene Symbol: LIPH
• Gene ID (NCBI): 200879
• RRID: AB_2135352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).

Publications

Species	Application	Title
human	WB	Genet Med; Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis.; Authors - Liron Malki
human	IHC	Cell Oncol (Dordr); Genomic analysis and filtration of novel prognostic biomarkers based on metabolic and immune subtypes in pancreatic cancer; Authors - Guangyu Chen
human	WB	J Cell Mol Med; LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer.; Authors - Yixiao Zhang ; KD Validated
human	IHC	Tumour Biol; Lipase member H is a novel secreted protein associated with a poor prognosis for breast cancer patients.; Authors - Meizi Cui
human	ELISA,WB,IHC	Biochem Biophys Res Commun; Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas.; Authors - Yasuhiro Seki
human	IHC	Tumour Biol; Lipase member H frequently overexpressed in human esophageal adenocarcinomas.; Authors - Hisako Ishimine