LIPG Monoclonal antibody, PBS Only

LIPG Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10B10

Cat no : 67434-1-PBS

Synonyms

EDL, EL, Endothelial lipase, lipase, endothelial, LIPG, PRO719

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Validation Data Gallery

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  WB analysis using 67434-1-Ig (same clone as 67434-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67434-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 67434-1-Ig (same clone as 67434-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67434-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67434-1-Ig (same clone as 67434-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67434-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HaCaT using 67434-1-Ig (same clone as 67434-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67434-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 67434-1-Ig (same clone as 67434-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67434-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67434-1-Ig (same clone as 67434-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67434-1-Ig (LIPG antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67434-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67434-1-Ig (same clone as 67434-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67434-1-Ig (LIPG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67434-1-PBS in a different storage buffer formulation.

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Product Information

67434-1-PBS targets LIPG in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LIPG fusion protein Ag29482
• Full Name: lipase, endothelial
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 and 42 kDa
• GenBank Accession Number: BC060825
• Gene Symbol: LIPG
• Gene ID (NCBI): 9388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.