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WB analysis of MKN-45 using 25889-1-AP
MKN-45 cells were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 25889-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 25889-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human stomach using 25889-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human stomach using 25889-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human stomach using 25889-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 25889-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 25889-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human stomach using 25889-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human stomach using 25889-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 25889-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 25889-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

LIPF Polyclonal antibody

Name: LIPF Polyclonal antibody
Brand: Proteintech
SKU: 25889-1-AP

LIPF Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25889-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

lipase, gastric, HLAL, HGL, GL, Gastric lipase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	MKN-45 cells, mouse stomach tissue
Positive IHC detected in	human stomach tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25889-1-AP targets LIPF in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LIPF fusion protein Ag23149 Predict reactive species
Full Name	lipase, gastric
Observed Molecular Weight	49 kDa
GenBank Accession Number	BC112272
Gene Symbol	LIPF
Gene ID (NCBI)	8513
RRID	AB_2880284
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07098
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

gastric lipase, is an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions.