LIPF Polyclonal antibody

LIPF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25889-1-AP

Synonyms

lipase, gastric, HLAL, HGL, GL, Gastric lipase

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Validation Data Gallery

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  WB analysis of MKN-45 using 25889-1-AP

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: MKN-45 cells, mouse stomach tissue
• Positive IHC detected in: human stomach tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25889-1-AP targets LIPF in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIPF fusion protein Ag23149
• Full Name: lipase, gastric
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC112272
• Gene Symbol: LIPF
• Gene ID (NCBI): 8513
• RRID: AB_2880284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

gastric lipase, is an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions.