HSL Polyclonal antibody

HSL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 17333-1-AP

Synonyms

LIPE, REH, LHS, Hormone-sensitive lipase, EC:3.1.1.79

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Validation Data Gallery

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  WB analysis using 17333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17333-1-AP (HSL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brown adipose using 17333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using 17333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse adipose tissue, pig adipose tissue
• Positive IHC detected in: mouse brown adipose tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

17333-1-AP targets HSL in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSL fusion protein Ag11360
• Full Name: lipase, hormone-sensitive
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC070041
• Gene Symbol: HSL
• Gene ID (NCBI): 3991
• RRID: AB_2878386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.

Publications

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Paternal cadmium exposure affects testosterone synthesis by reducing the testicular cholesterol pool in offspring mice; Authors - Jinzhao Zhou
	WB	Int J Biol Sci; LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.; Authors - Yan Fang
human	WB,IF,IP	Biochim Biophys Acta Mol Cell Biol Lipids; DECR1 directly activates HSL to promote lipolysis in cervical cancer cells.; Authors - Huijuan Zhou
mouse		Biochim Biophys Acta Mol Cell Biol Lipids; PLK1 promotes cholesterol efflux and alleviates atherosclerosis by up-regulating ABCA1 and ABCG1 expression via the AMPK/PPARγ/LXRα pathway; Authors - Heng-Jing Hu
pig	WB	Epigenetics; Differential regulatory roles of microRNAs during intramuscular adipogenesis in Chinese Guizhou Congjiang Xiang pigs.; Authors - Lulin Tan
mouse	WB	Food Funct; Gentisic acid prevents diet-induced obesity in mice by accelerating the thermogenesis of brown adipose tissue.; Authors - Xue Han