LIN54 Polyclonal antibody

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  WB analysis using 26644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26644-1-AP (LIN54 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

LIN54 Polyclonal Antibody for WB, ELISA

Cat No. 26644-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CXCDC1, JC8.6, KIAA2037, lin 54 homolog (C. elegans), LIN54, MIP120, Protein lin 54 homolog

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26644-1-AP targets LIN54 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIN54 fusion protein Ag24507
• Full Name: lin-54 homolog (C. elegans)
• Calculated Molecular Weight: 749 aa, 79 kDa
• Observed Molecular Weight: 79 kDa, 100 kDa
• GenBank Accession Number: BC109278
• Gene Symbol: LIN54
• Gene ID (NCBI): 132660
• RRID: AB_3669558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6MZP7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIN54 is a component of the LINC/DREAM complex, an important regulator of cell cycle genes (PMID: 17671431; 19725879). The complex binds to more than 800 promoters in G0 phase and is required for repression of E2F target genes (PMID:17531812). In S phase, the complex binds to the promoters of G2/M genes whose products are required for mitosis and plays an important role in their cell cycle dependent activation (PMID:17671431). LIN54 has a DNA binding region (CXC-hinge-CXC, CHC domain), which consists of two cysteine-rich (CXC) domains separated by a short spacer. It has been reported that the CHC domain functions as a DNA binding domain, and conserved cysteine residues in two CXC domains are essential for DNA binding (PMID: 22895175). The calculated molecular weight of LIN54 is 79 kDa and an apparent molecular mass of 100-110 kDa has been reported (PMID: 17531812; 28920576; 17671431).

Publications

Species	Application	Title
	WB	Int J Oncol; Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9; Authors - Zhenwei Ma