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  • Western Blot (WB) analysis of various lysates using LIN54 Polyclonal antibody (26644-1-AP)

    WB analysis using 26644-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26644-1-AP (LIN54 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

LIN54 Polyclonal antibody

LIN54 Polyclonal Antibody for WB, ELISA
Cat No. 26644-1-AP
Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CXCDC1, JC8.6, KIAA2037, lin 54 homolog (C. elegans), LIN54, MIP120, Protein lin 54 homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26644-1-AP targets LIN54 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIN54 fusion protein Ag24507 Predict reactive species
Full Name lin-54 homolog (C. elegans)
Calculated Molecular Weight 749 aa, 79 kDa
Observed Molecular Weight79 kDa, 100 kDa
GenBank Accession NumberBC109278
Gene Symbol LIN54
Gene ID (NCBI) 132660
RRIDAB_3669558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6MZP7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIN54 is a component of the LINC/DREAM complex, an important regulator of cell cycle genes (PMID: 17671431; 19725879). The complex binds to more than 800 promoters in G0 phase and is required for repression of E2F target genes (PMID:17531812). In S phase, the complex binds to the promoters of G2/M genes whose products are required for mitosis and plays an important role in their cell cycle dependent activation (PMID:17671431). LIN54 has a DNA binding region (CXC-hinge-CXC, CHC domain), which consists of two cysteine-rich (CXC) domains separated by a short spacer. It has been reported that the CHC domain functions as a DNA binding domain, and conserved cysteine residues in two CXC domains are essential for DNA binding (PMID: 22895175). The calculated molecular weight of LIN54 is 79 kDa and an apparent molecular mass of 100-110 kDa has been reported (PMID: 17531812; 28920576; 17671431).

Protocols

Product Specific Protocols
WB protocol for LIN54 antibody 26644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Int J Oncol

Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9

Authors - Zhenwei Ma
View Article