LIN28B Polyclonal antibody

LIN28B Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA, PLA

Conjugate

Unconjugated

Publications

4

Cat no : 24017-1-AP

Synonyms

Protein lin 28 homolog B, Lin-28B, Lin28, Lin 28B, lin 28 homolog B (C. elegans)

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Validation Data Gallery

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  WB analysis using 24017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24017-1-AP (LIN28B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24017-1-AP

  IP result of anti-LIN28B (IP:24017-1-AP, 4ug; Detection:24017-1-AP 1:500) with HEK-293 cells lysate 1480ug.

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  IF Staining of NCCIT using 24017-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using LIN28B antibody (24017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: NCCIT cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

24017-1-AP targets LIN28B in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIN28B fusion protein Ag21241
• Full Name: lin-28 homolog B (C. elegans)
• Calculated Molecular Weight: 250 aa, 27 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC137526
• Gene Symbol: LIN28B
• Gene ID (NCBI): 389421
• RRID: AB_2879403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. The calculated molecular weight of LIN28B is 27 kDa, but a 35 kDa band has been detected in some articles (PMID: 33029224, 33378003).

Publications

Species	Application	Title
mouse	IF	Front Immunol; Lin28 regulates thymic growth and involution and correlates with MHCII expression in thymic epithelial cells; Authors - Shiyun Xiao
human	WB,PLA	iScience; Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells; Authors - Nam-Kyung Yu
mouse	WB	Pharm Biol; Peptides from the croceine croaker (Larimichthys crocea) swim bladder attenuate busulfan-induced oligoasthenospermia in mice.; Authors - Xi Zhao
human	IP, IHC	Int J Clin Exp Pathol; Lin28B is a novel prognostic marker in gastric adenocarcinoma.; Authors - Qian Hu