LIN28A Monoclonal antibody, PBS Only

LIN28A Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F5G6

Cat no : 60344-1-PBS

Synonyms

LIN28, Lin-28A, Lin 28A, LIN 28, CSDD1

---

Validation Data Gallery

• 

  WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using LIN28 antibody (60344-1-Ig, Clone: 4F5G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

---

Product Information

60344-1-PBS targets LIN28A in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LIN28A fusion protein Ag2312
• Full Name: lin-28 homolog (C. elegans)
• Calculated Molecular Weight: 209 aa, 23 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC028566
• Gene Symbol: LIN28
• Gene ID (NCBI): 79727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.