LIMK2 Polyclonal antibody

LIMK2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 12350-1-AP

Synonyms

LIM domain kinase 2, LIMK 2, LIMK2

---

Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 12350-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12350-1-AP (LIMK2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human stomach using 12350-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12350-1-AP (LIMK2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

12350-1-AP targets LIMK2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIMK2 fusion protein Ag3011
• Full Name: LIM domain kinase 2
• Calculated Molecular Weight: 686 aa, 78 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC013051
• Gene Symbol: LIMK2
• Gene ID (NCBI): 3985
• RRID: AB_2137094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The family of LIM kinase (LIMK) includes two similar proteins, LIMK1 and LIMK2, that share overall identity of 53% with 75%, 55%, and 42% amino acid identity in the LIM, kinase, and PDZ domains, respectively. LIMK2 regulates actin cytoskeletal reorganization through phosphorylating and inactivating cofilin, an actin-depolymerizing factor of actin filaments. It also may associate with gamma-tubulin and play a role in mitotic spindle assembly. High levels of LIMK2 are evident in uterus, thymus, and spleen whereas lower levels are observed in brain, heart, liver, stomach, skeletal muscle, and testes according to the results of western blotting(PMID:16399995). It has 3 isoforms produced by alternative splicing of the molecular weight of 70-78 kDa. It can be detected a band of 47 kDa or 50-55 kDa in the testes because the antibody is recognizing the testis-specific variant of LIMK2 and the this difference could be due to species variation(PMID:17928627).

Publications

Species	Application	Title
human	WB	Adv Healthc Mater; Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.; Authors - Moyuan Deng
human	IF	J Cachexia Sarcopenia Muscle; RhoA/ROCK2 signalling is enhanced by PDGF-AA in fibro-adipogenic progenitor cells: implications for Duchenne muscular dystrophy.; Authors - Esther Fernández-Simón
human	IHC	Theranostics; NUDT21 inhibits bladder cancer progression through ANXA2 and LIMK2 by alternative polyadenylation.; Authors - Ming Xiong
human	WB,IHC	Cell Death Dis; Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.; Authors - Yue Zhang
mouse	WB	PLoS One; ECM-dependent HIF induction directs trophoblast stem cell fate via LIMK1-mediated cytoskeletal rearrangement.; Authors - Choi Hwa J HJ
human	WB,IF	Mol Biotechnol; Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling; Authors - Naiyi Du