LIMK1 Polyclonal antibody

LIMK1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 19699-1-AP

Synonyms

LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1

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Validation Data Gallery

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  IHC staining of human heart using 19699-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19699-1-AP (LIMK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 19699-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19699-1-AP (LIMK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 19699-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19699-1-AP (LIMK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 19699-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19699-1-AP (LIMK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 19699-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LIMK1 antibody (19699-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive IHC detected in: human heart tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

19699-1-AP targets LIMK1 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: LIM domain kinase 1
• Calculated Molecular Weight: 73 kDa
• GenBank Accession Number: NM_002314
• Gene Symbol: LIMK1
• Gene ID (NCBI): 3984
• RRID: AB_10696174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.

Publications

Species	Application	Title
human		Cell Death Dis; Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.; Authors - Yue Zhang
mouse		Commun Biol; RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling; Authors - Haixia Zhu
human	IHC	Oncol Rep; FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression.; Authors - Xiufang Yang
human		Cancer Med; MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.; Authors - Nengquan Sheng
human		Cell Death Discov; FMNL2 suppresses cell migration and invasion of breast cancer: a reduction of cytoplasmic p27 via RhoA/LIMK/Cofilin pathway.; Authors - Xinyan Jiao
human		Mol Carcinog; LKB1 loss promotes colorectal cancer cell metastasis through regulating TNIK expression and actin cytoskeleton remodeling; Authors - Guanghui Hu