LIMK1 Polyclonal antibody

LIMK1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 19699-1-AP

Synonyms

LIM domain kinase 1, LIMK, LIMK 1, LIMK1



Tested Applications

Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19699-1-AP targets LIMK1 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name LIM domain kinase 1
Calculated Molecular Weight 73 kDa
GenBank Accession NumberNM_002314
Gene Symbol LIMK1
Gene ID (NCBI) 3984
RRIDAB_10696174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.

Protocols

Product Specific Protocols
IHC protocol for LIMK1 antibody 19699-1-APDownload protocol
IF protocol for LIMK1 antibody 19699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell Death Dis

Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.

Authors - Yue Zhang
mouse

Commun Biol

RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling

Authors - Haixia Zhu
humanIHC

Oncol Rep

FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression.

Authors - Xiufang Yang
human

Cancer Med

MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.

Authors - Nengquan Sheng
human

Cell Death Discov

FMNL2 suppresses cell migration and invasion of breast cancer: a reduction of cytoplasmic p27 via RhoA/LIMK/Cofilin pathway.

Authors - Xinyan Jiao
human

Mol Carcinog

LKB1 loss promotes colorectal cancer cell metastasis through regulating TNIK expression and actin cytoskeleton remodeling

Authors - Guanghui Hu