Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody, PBS Only

LIGHT/CD258 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240694D7

Cat no : 98063-1-PBS

Synonyms

LIGHT, HVEM-L, HVEML, HVEM L, CD258

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Validation Data Gallery

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  FC experiment of human PBMCs using 98063-1-RR (same clone as 98063-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR, Clone:240694D7) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were then stained with APC Anti-Human CD8a. Cells were not fixed. This data was developed using the same antibody clone with 98063-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98063-1-RR (same clone as 98063-1-PBS)

  1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated (red) human PBMCs were stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR, Clone:240694D7) and PE-Conjugated Goat Anti-Rabbit IgG (H+L), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98063-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98063-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98063-1-RR against Human LIGHT/CD258 were performed. The affinity constant is 1.25 nM.

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Product Information

The immunogen of 98063-1-PBS is LIGHT/CD258 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: tumor necrosis factor (ligand) superfamily, member 14
• Calculated Molecular Weight: 26KD
• GenBank Accession Number: NM_003807
• Gene Symbol: LIGHT
• Gene ID (NCBI): 8740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.