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Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody

LIGHT/CD258 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240694D7

Cat no : 98063-1-RR

Synonyms

LIGHT, HVEM-L, HVEML, HVEM L, CD258

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR)

    FC experiment of human PBMCs using 98063-1-RR

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR, Clone:240694D7) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were then stained with APC Anti-Human CD8a. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR)

    FC experiment of human PBMCs using 98063-1-RR

    1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated (red) human PBMCs were stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR, Clone:240694D7) and PE-Conjugated Goat Anti-Rabbit IgG (H+L), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98063-1-RR

    Affinity and Kinetic Characterization of 98063-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98063-1-RR against Human LIGHT/CD258 were performed. The affinity constant is 1.25 nM.

Tested Applications

Positive FC detected inPMA, Ionomycin and Brefeldin A treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98063-1-RR is LIGHT/CD258 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name tumor necrosis factor (ligand) superfamily, member 14
Calculated Molecular Weight26KD
GenBank Accession NumberNM_003807
Gene Symbol LIGHT
Gene ID (NCBI) 8740
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.

Protocols

Product Specific Protocols
FC protocol for LIGHT/CD258 antibody 98063-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols