LIG3 Polyclonal antibody

• Featured Product
• KD/KO Validated

LIG3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 26583-1-AP

Synonyms

ligase III, DNA, ATP dependent, LIG2, EC:6.5.1.1, DNA ligase III, DNA ligase 3

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Validation Data Gallery

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  WB analysis of HEK-293T using 26583-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26583-1-AP (LIG3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26583-1-AP (LIG3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 26583-1-AP

  IP result of anti-LIG3 (IP:26583-1-AP, 4ug; Detection:26583-1-AP 1:300) with HEK-293T cells lysate 4000ug.

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  IHC staining of mouse testis using 26583-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26583-1-AP (LIG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 26583-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26583-1-AP (LIG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26583-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LIG3 antibody (26583-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, PC-3 cells
• Positive IP detected in: HEK-293T cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

26583-1-AP targets LIG3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIG3 fusion protein Ag24998
• Full Name: ligase III, DNA, ATP-dependent
• Calculated Molecular Weight: 100-110 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC068005
• Gene Symbol: LIG3
• Gene ID (NCBI): 3980
• RRID: AB_2880562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA ligase 3 is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.

Publications

Species	Application	Title
human	IF	Brain; Biallelic variants in LIG3 cause a novel mitochondrial neurogastrointestinal encephalomyopathy.; Authors - Elena Bonora
pig	WB	Int J Biochem Cell Biol; Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.; Authors - Guoling Li
human	WB	bioRxiv; Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy; Authors - Zhihua Kang
	WB	bioRxiv; D-type cyclins regulate DNA mismatch repair in the G1 and S phases of the cell cycle, maintaining genome stability; Authors - Gergely Rona
human	WB	Mol Cell; CDK-independent role of D-type cyclins in regulating DNA mismatch repair; Authors - Gergely Rona
human	WB	Nature; Retrotransposons hijack alt-EJ for DNA replication and eccDNA biogenesis; Authors - Fu Yang ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

MANOHAR (Verified Customer) (12-11-2019)

No nonspecific binding

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T