LIG1 Monoclonal antibody

LIG1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3D9G8

Cat no : 67840-1-Ig

Synonyms

DNA ligase 1, DNA ligase I, LIG1, ligase I, DNA, ATP dependent

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Validation Data Gallery

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  WB analysis using 67840-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67840-1-Ig (LIG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• CoIP: See 1 publications below

Product Information

67840-1-Ig targets LIG1 in WB, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: LIG1 fusion protein Ag12489
• Full Name: ligase I, DNA, ATP-dependent
• Calculated Molecular Weight: 919 aa, 102 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC108318
• Gene Symbol: LIG1
• Gene ID (NCBI): 3978
• RRID: AB_2918602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 67840-1-Ig is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.

Publications

Species	Application	Title
human	WB,CoIP	bioRxiv; Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy; Authors - Zhihua Kang