LIAS Monoclonal antibody, PBS Only

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  WB analysis of LNCaP using 67298-1-Ig (same clone as 67298-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67298-1-Ig (same clone as 67298-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67298-1-Ig (same clone as 67298-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67298-1-Ig (LIAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

LIAS Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67298-1-PBS

Clone No.3B5G7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

3B5G7, EC:2.8.1.8, HUSSY 01, HUSSY-01, LAS

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Product Information

67298-1-PBS targets LIAS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27286

  Product name: Recombinant human LIAS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-372 aa of BC023635

  Sequence: MSLRCGDAARTLGPRVFGRYFCSPVRPLSSLPDKKKELLQNGPDLQDFVSGDLADRSTWDEYKGNLKRQKGERLRLPPWLKTEIPMGKNYNKLKNTLRNLNLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDASEPYNTAKAIAEWGLDYVVLTSVDRDDMPDGGAEHIAKTVSYLKERNPKILVECLTPDFRGDLKAIEKVALSGLDVYAHNVETVPELQSKVRDPRVNFDQSLRVLKHAKKVQPDVISKTSIMLGLGENDEQVYATMKALREADVDCLTLGQYMQPTRRHLKVEEYITPEKFKYWEKVGNELGFHYTASGPLVRSSYKAGEFFLKNLVAKRKTKDL

• Full Name: lipoic acid synthetase
• Calculated Molecular Weight: 372 aa, 42 kDa
• Observed Molecular Weight: 34-42 kDa
• GenBank Accession Number: BC023635
• Gene Symbol: LIAS
• Gene ID (NCBI): 11019
• RRID: AB_2882562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O43766
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).