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LIAS Monoclonal antibody

LIAS Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B5G7

Cat no : 67298-1-Ig

Synonyms

HUSSY 01, LAS, LIAS, Lip syn, LIP1, Lipoate synthase, Lipoic acid synthase, lipoic acid synthetase, Lipoyl synthase, mitochondrial, LS

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using LIAS Monoclonal antibody (67298-1-Ig)

    WB analysis of LNCaP using 67298-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LIAS Monoclonal antibody (67298-1-Ig)

    WB analysis using 67298-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LIAS Monoclonal antibody (67298-1-Ig)

    IHC staining of mouse brain using 67298-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67298-1-Ig (LIAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, pig brain tissue, HEK-293 cells, K-562 cells, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67298-1-Ig targets LIAS in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LIAS fusion protein Ag27286
Full Name lipoic acid synthetase
Calculated Molecular Weight 372 aa, 42 kDa
Observed Molecular Weight 34-42 kDa
GenBank Accession NumberBC023635
Gene Symbol LIAS
Gene ID (NCBI) 11019
RRIDAB_2882562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).

Protocols

Product Specific Protocols
WB protocol for LIAS antibody 67298-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols