LGR5 Polyclonal antibody

LGR5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 30007-1-AP

Synonyms

G-protein coupled receptor HG38, G-protein coupled receptor 67, G-protein coupled receptor 49, GPR67, GPR49

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Validation Data Gallery

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  WB analysis using 30007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30007-1-AP (LGR5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

30007-1-AP targets LGR5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LGR5 fusion protein Ag31625
• Full Name: leucine-rich repeat-containing G protein-coupled receptor 5
• Calculated Molecular Weight: 907 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC096324
• Gene Symbol: LGR5
• Gene ID (NCBI): 8549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67) is a protein that in humans is encoded by the LGR5 gene. It is a member of GPCR class A receptor proteins. R-spondin proteins are the biological ligands of LGR5. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.LGR5 is crucial during embryogenesis as LGR null studies in mice incurred 100% neonatal mortality accompanied by several craniofacial distortions such as ankyloglossia and gastrointestinal dilation (PMID: 9642114, 16575208, 525477).

Publications

Species	Application	Title
mouse	IHC	Burns Trauma; Nocardia rubra cell-wall skeleton mitigates whole abdominal irradiation-induced intestinal injury via regulating macrophage function; Authors - Lingling Wu
mouse	IF	Acta Histochem; Stimulation of mouse hair regrowth by exosomes derived from human umbilical cord mesenchymal stem cells; Authors - Yang Jiao