LGALS9/Galectin-9 Polyclonal antibody

• Featured Product
• KD/KO Validated

LGALS9/Galectin-9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 17938-1-AP

Synonyms

Galectin 9, LGALS9, HUAT, Gal-9, Gal 9

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Validation Data Gallery

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  WB analysis of U-937 using 17938-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 17938-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17938-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17938-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17938-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of U-937 using 17938-1-AP

  IP result of anti-Galectin-9 (IP:17938-1-AP, 4ug; Detection:17938-1-AP 1:1500) with U-937 cells lysate 2240 ug.

• 

  IHC staining of human ovary cancer using 17938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17938-1-AP (Galectin-9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17938-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 17938-1-AP (Galectin 9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: U-937 cells, HeLa cells, HepG2 cells, HL-60 cells, SGC-7901 cells
• Positive IP detected in: U-937 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

17938-1-AP targets LGALS9/Galectin-9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LGALS9/Galectin-9 fusion protein Ag12209
• Full Name: lectin, galactoside-binding, soluble, 9
• Calculated Molecular Weight: 323 aa, 36 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC110340
• Gene Symbol: Galectin-9
• Gene ID (NCBI): 3965
• RRID: AB_2137233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.

Publications

Species	Application	Title
	WB	Front Mol Neurosci; Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease; Authors - Qinyu Peng
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	WB,IHC	Front Immunol; Galectin-9/TIM-3 as a Key Regulator of Immune Response in Gliomas With Chromosome 1p/19q Codeletion.; Authors - Guanzhang Li
human	WB,IHC	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
human	WB	Stem Cell Res Ther; Mesenchymal stem cells alleviate experimental autoimmune cholangitis through immunosuppression and cytoprotective function mediated by galectin-9.; Authors - Junyu Fan
human	WB,IHC,IP	Immunotherapy; Galectin-9 and PD-L1 antibody blockade combination therapy inhibits tumour progression in pancreatic cancer; Authors - Enliang Li