LEPREL2 Monoclonal antibody, PBS Only

LEPREL2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F6F3

Cat no : 68299-1-PBS

Synonyms

GRCB, HSU47926, leprecan like 2, Leprecan like protein 2, LEPREL2, P3H3, Prolyl 3 hydroxylase 3, Protein B

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Validation Data Gallery

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  WB analysis of HeLa using 68299-1-Ig (same clone as 68299-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 68299-1-Ig (same clone as 68299-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 68299-1-Ig (same clone as 68299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 68299-1-Ig (LEPREL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

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Product Information

68299-1-PBS targets LEPREL2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LEPREL2 fusion protein Ag8850
• Full Name: leprecan-like 2
• Calculated Molecular Weight: 736 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC017217
• Gene Symbol: LEPREL2
• Gene ID (NCBI): 10536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.