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P3H3 Polyclonal antibody
P3H3 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 16023-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse ovary tissue, MDA-MB-453s cells, rat ovary tissue |
| Positive IP detected in | MDA-MB-453s cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IP | See 1 publications below |
Product Information
16023-1-AP targets P3H3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | P3H3 fusion protein Ag8850 |
| Full Name | leprecan-like 2 |
| Calculated Molecular Weight | 736 aa, 82 kDa |
| Observed Molecular Weight | 82-90 kDa |
| GenBank Accession Number | BC017217 |
| Gene Symbol | LEPREL2 |
| Gene ID (NCBI) | 10536 |
| RRID | AB_2281291 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P3H3 antibody 16023-1-AP | Download protocol |
| IP protocol for P3H3 antibody 16023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Genet Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation. | ||
PLoS Genet Cyclophilin B control of lysine post-translational modifications of skin type I collagen. | ||
Sci Rep Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome and endothelial function estimates. | ||
J Biol Chem P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.
| ||
J Biol Chem Type I and type V procollagen triple helix use different subsets of the molecular ensemble for lysine post-translational modifications in the rER. | ||
AIMS Genet Expression characterization and functional implication of the collagen-modifying Leprecan proteins in mouse gonadal tissue and mature sperm. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Boyan (Verified Customer) (12-16-2021) | This antibody did not detect any band in RPE1 or MEF cell lysates
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