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  • KD/KO Validated

P3H3 Polyclonal antibody

P3H3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16023-1-AP

Synonyms

GRCB, HSU47926, leprecan like 2, Leprecan like protein 2, LEPREL2, P3H3, Prolyl 3 hydroxylase 3, Protein B



Tested Applications

Positive WB detected inmouse ovary tissue, MDA-MB-453s cells, rat ovary tissue
Positive IP detected inMDA-MB-453s cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16023-1-AP targets P3H3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P3H3 fusion protein Ag8850
Full Name leprecan-like 2
Calculated Molecular Weight 736 aa, 82 kDa
Observed Molecular Weight 82-90 kDa
GenBank Accession NumberBC017217
Gene Symbol LEPREL2
Gene ID (NCBI) 10536
RRIDAB_2281291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.

Protocols

Product Specific Protocols
WB protocol for P3H3 antibody 16023-1-APDownload protocol
IP protocol for P3H3 antibody 16023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

PLoS Genet

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.

Authors - Melissa E Heard
mouseWB

PLoS Genet

Cyclophilin B control of lysine post-translational modifications of skin type I collagen.

Authors - Masahiko Terajima
humanWB

Sci Rep

Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome and endothelial function estimates.

Authors - Bernd Stratmann
MOUSEWB

J Biol Chem

P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.

Authors - David M Hudson
  • KO Validated
mouseWB

J Biol Chem

Type I and type V procollagen triple helix use different subsets of the molecular ensemble for lysine post-translational modifications in the rER.

Authors - Yoshihiro Ishikawa
mouseWB

AIMS Genet

Expression characterization and functional implication of the collagen-modifying Leprecan proteins in mouse gonadal tissue and mature sperm.

Authors - Sarah M Zimmerman

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (12-16-2021)

This antibody did not detect any band in RPE1 or MEF cell lysates

  • Applications: Western Blot