P3H2 Polyclonal antibody

• Featured Product
• KD/KO Validated

P3H2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 15723-1-AP

Synonyms

leprecan like 1, Leprecan like protein 1, LEPREL1, MLAT4, P3H2, Prolyl 3 hydroxylase 2

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Validation Data Gallery

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  WB analysis using 15723-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15723-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15723-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15723-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 15723-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 15723-1-AP

  IP result of anti-P3H2 (IP:15723-1-AP, 4ug; Detection:15723-1-AP 1:600) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human liver using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15723-1-AP (P3H2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15723-1-AP (P3H2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15723-1-AP (P3H2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15723-1-AP (P3H2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: L02 cells, HEK-293 cells, mouse placenta tissue, mouse kidney tissue, human placenta tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human liver tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

15723-1-AP targets P3H2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P3H2 fusion protein Ag8444
• Full Name: leprecan-like 1
• Calculated Molecular Weight: 708aa,81 kDa; 527aa,60 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC005029
• Gene Symbol: LEPREL1
• Gene ID (NCBI): 55214
• RRID: AB_2136486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P3H2 (prolyl 3-hydroxylase 2, also known as LEPREL1) is a member of the leprecan family of proteins, which also include P3H1, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H2 shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations*; Authors - David M Hudson
human	WB	Sci Rep; Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure; Authors - Takahiro Isono
human	WB	J Biol Chem; Post-translational modification of type IV collagen with 3-hydroxyproline affects its interactions with glycoprotein VI and nidogens 1 and 2.; Authors - Nathan T Montgomery ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

We conducted a screening of basal expression levels in multiple human tumor cell lines, attempting antibody concentrations ranging from 1:500 to 2000, all of which exhibited clear bands, indicating stable working conditions. However, the expression depends on the cancer cell line itself.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Multiple cancer cell lines

P3H2 Antibody Western Blot validation (1:500 dilution) in Multiple cancer cell lines (Cat no:15723-1-AP)