LENG9 Polyclonal antibody, PBS Only

LENG9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16295-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 16295-1-AP (same clone as 16295-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 16295-1-AP (same clone as 16295-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16295-1-AP (same clone as 16295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16295-1-AP (same clone as 16295-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LENG9 antibody (16295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 16295-1-AP (same clone as 16295-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using LENG9 antibody 16295-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

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Product Information

16295-1-PBS targets LENG9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LENG9 fusion protein Ag9481
• Full Name: leukocyte receptor cluster (LRC) member 9
• Calculated Molecular Weight: 501 aa, 53 kDa
• Observed Molecular Weight: 54-60 kDa
• GenBank Accession Number: BC015921
• Gene Symbol: LENG9
• Gene ID (NCBI): 94059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LENG9 is rarely reported in the literature. GO function analysis revealed that the molecular function of LENG9 is metal ion binding (GO:0046872), and the BioGRID database showed that LENG9 has 6 interactors, including C9ORF41, B4GALT2, CDC5L, D2HGDH, FOXS1 and OGT. Studies have shown that LENG9 may play an oncogenic role in tumor pathogenesis by promoting tumor cell proliferation, migration, and invasion. (PMID: 35538075)