LEFTY2 Polyclonal antibody

LEFTY2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13991-1-AP

Synonyms

Protein lefty-2, LEFTY 2, Left-right determination factor A, Left-right determination factor 2, left right determination factor 2

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Validation Data Gallery

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  WB analysis of HeLa using 13991-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13991-1-AP (LEFTY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 13991-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 13991-1-AP (LEFTY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human endometrial cancer using 13991-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13991-1-AP (LEFTY2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 13991-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13991-1-AP (LEFTY2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13991-1-AP

  Immunofluorescent analysis of HeLa cells using 13991-1-AP (LEFTY2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse uterus tissue
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13991-1-AP targets LEFTY2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LEFTY2 fusion protein Ag5073
• Full Name: left-right determination factor 2
• Calculated Molecular Weight: 366 aa, 41 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC035718
• Gene Symbol: LEFTY2
• Gene ID (NCBI): 7044
• RRID: AB_2135782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Stroke; Mesenchymal Behavior of the Endothelium Promoted by SMAD6 Downregulation Is Associated With Brain Arteriovenous Malformation Microhemorrhage.; Authors - Weilun Fu
mouse	WB	J Biol Chem; MicroRNA-127 Promotes Mesendoderm Differentiation of Mouse Embryonic Stem Cells by Targeting Left-Right Determination Factor 2.; Authors - Haixia Ma
rat	IHC	Dev Biol; Role of EBAF/Nodal/p27 signaling pathway in development of placenta in normal and diabetic rats.; Authors - Qianqian Gao