LEFTY1 Recombinant antibody

LEFTY1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240346E11

Cat no : 83586-1-RR

Synonyms

LEFTB, LEFTY1, LEFTYB, Protein lefty 1, Protein lefty B

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Validation Data Gallery

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  WB analysis using 83586-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83586-1-RR (LEFTY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat heart tissue, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83586-1-RR is LEFTY1 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LEFTY1 fusion protein Ag4925
• Full Name: left-right determination factor 1
• Calculated Molecular Weight: 366 aa, 41 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC027883
• Gene Symbol: LEFTY1
• Gene ID (NCBI): 10637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lefty is a member of the transforming growth factor (TGF) β superfamily, which is implicated in left-right patterning during embryogenesis. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans.