LDLR Recombinant antibody, PBS Only

LDLR Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1O11

Cat no : 82724-1-PBS

Synonyms

LDL receptor, FHC, FH, 1O11

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Validation Data Gallery

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  WB analysis using 82724-1-RR (same clone as 82724-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82724-1-RR (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82724-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82724-1-RR (same clone as 82724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82724-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82724-1-RR (same clone as 82724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82724-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82724-1-RR (same clone as 82724-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDLR antibody (82724-1-RR, Clone: 1O11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82724-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82724-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82724-1-RR against Human LDLR were performed. The affinity constant is below 1 pM.

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Product Information

82724-1-PBS targets LDLR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LDLR fusion protein Ag1236
• Full Name: low density lipoprotein receptor
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: BC014514
• Gene Symbol: LDLR
• Gene ID (NCBI): 3949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.