LDHC Polyclonal antibody

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  WB analysis using 14546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14546-1-AP (LDHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-231 using 14546-1-AP

  WB result of LDHC antibody (14546-1-AP; 1:800; room temperature for 1.5 hours) with wild-type and LDHC knockout MDA-MB-231 cells.

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  IHC staining of mouse testis using 14546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14546-1-AP (LDHC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14546-1-AP (LDHC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14546-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDHC antibody (14546-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

LDHC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14546-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

LDH-C, LDH3, LDH X, LDH C, LDH

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Tested Applications

• Positive WB detected in: HeLa cells, MDA-MB-231 cells, PC-3 cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14546-1-AP targets LDHC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LDHC fusion protein Ag6050
• Full Name: lactate dehydrogenase C
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 31-35 kDa
• GenBank Accession Number: BC064388
• Gene Symbol: LDHC
• Gene ID (NCBI): 3948
• RRID: AB_3085437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07864
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.