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LDHB Recombinant antibody, PBS Only

LDHB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1M20

Cat no : 81963-1-PBS

Synonyms

lactate dehydrogenase B, LDH H, LDH B, LDH, EC:1.1.1.27

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LDHB Recombinant antibody (81963-1-RR)

    WB analysis using 81963-1-RR (same clone as 81963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81963-1-RR (LDHB antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using LDHB Recombinant antibody (81963-1-RR)

    IP experiment of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

    IP result of anti-LDHB (IP:81963-1-RR, 4ug; Detection:81963-1-RR 1:50000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LDHB Recombinant antibody (81963-1-RR)

    IF Staining of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LDHB antibody (81963-1-RR, Clone: 1M20 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using LDHB Recombinant antibody (81963-1-RR)

    FC experiment of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LDHB (81963-1-RR, Clone:1M20) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

Product Information

81963-1-PBS targets LDHB in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LDHB fusion protein Ag6605
Full Name lactate dehydrogenase B
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002362
Gene Symbol LDHB
Gene ID (NCBI) 3945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway. LDH comprises LDHA and LDHB, two subunits that are encoded by independent genes. In muscle or liver, most of the LDH is composed of four LDHA subunits, and preferably catalyzes the reduction of pyruvate to lactate. In heart and brain, LDH is mainly composed of four LDHB subunits, and predominantly catalyzes the oxidation of lactate to pyruvate. In other tissues, LDH is composed of both LDHA and LDHB. (PMID: 26269128, 31804482)