LDHB Monoclonal antibody, PBS Only

LDHB Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5E9G1

Cat no : 66425-1-PBS

Synonyms

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, LDH-B, TRG 5

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Validation Data Gallery

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  WB analysis using 66425-1-Ig (same clone as 66425-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66425-1-Ig (LDHB antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66425-1-Ig (same clone as 66425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66425-1-Ig (LDHB antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66425-1-Ig (same clone as 66425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66425-1-Ig (LDHB antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66425-1-Ig (same clone as 66425-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66425-1-Ig (LDHB antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66425-1-PBS targets LDHB in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

• Tested Reactivity: human, rat, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LDHB fusion protein Ag6605
• Full Name: lactate dehydrogenase B
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002362
• Gene Symbol: LDHB
• Gene ID (NCBI): 3945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.