LDHA-Specific Recombinant antibody, PBS Only

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  WB analysis using 84198-2-RR (same clone as 84198-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84198-2-RR (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-231 using 84198-2-RR (same clone as 84198-2-PBS)

  WB result of LDHA-Specific antibody (84198-2-RR; 1:3000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 84198-2-RR (same clone as 84198-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA-Specific Recombinant antibody (84198-2-RR, Clone:240885A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

LDHA-Specific Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 84198-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

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Freight/Packing: -

Quantity

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Product Information

84198-2-PBS targets LDHA-Specific in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: lactate dehydrogenase A
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_005566
• Gene Symbol: LDHA
• Gene ID (NCBI): 3939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P00338
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.