LBP Recombinant antibody

LBP Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230524G10

Cat no : 83427-1-RR

Synonyms

Lipopolysaccharide-binding protein, 230524G10

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Validation Data Gallery

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  WB analysis of A431 using 83427-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 83427-1-RR (LBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83427-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83427-1-RR against Human LBP were performed. The affinity constant is 27.1 nM.

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Tested Applications

• Positive WB detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83427-1-RR is LBP Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: lipopolysaccharide binding protein
• Calculated Molecular Weight: 53KD
• Observed Molecular Weight: 63-67 kDa
• GenBank Accession Number: NM_004139.4
• Gene Symbol: LBP
• Gene ID (NCBI): 3929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)